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Occurence of antibodies against chlamydial lipopolysaccharide in human sera as measured by ELISA using an artificial glycoconjugate antigen

Lore Brade, Helga Brunnemann, Martin Ernst, Yucai Fu, Otto Holst, Paul Kosma, Helmut Näher, Kenneth Persson, Helmut Brade
DOI: http://dx.doi.org/10.1111/j.1574-695X.1994.tb00422.x 27-41 First published online: 1 January 1994

Abstract

An artificial glycoconjugate containing, as a ligand, the deacylated carbohydrate backbone of a recombinant Chlamydia specific lipopolysaccharide was used as a solid-phase antigen in ELISA to measure antibodies against chlamydial LPS. The specificity and reproducibility of the assay was shown by using a panel of prototype monoclonal antibodies representing the spectrum of antibodies also occurring in patient sera. These mAbs recognized Chlamydia-specific epitopes [α2 →8-linked disaccharide of 3-deoxy-D-manno-octulosonic acid (Kdo) or the trisaccharide αKdo-(2 → 8)-αKdo-(2 → 4)-αKdo] or those shared between chlamydial and Re-type LPS (αKdo, α2 → 4-linked Kdo disaccharide). The assay was used to measure IgG, IgA and IgM antibodies against chlamydial LPS in patients with genital or respiratory tract infections. In comparison to the results obtained with sera from blood donors, it became evident that both types of infection result in significant changes in the profile of LPS antibodies.

Key words
  • Chlamydiae
  • LPS
  • LPS antibodies
  • Artificial antigen
  • ELISA
  • Diagnosis

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